Mops
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers.
It is a structural analog to MES, and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
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| Names | |
|---|---|
| Preferred IUPAC name 3-(Morpholin-4-yl)propane-1-sulfonic acid | |
| Other names 3-(N-Morpholino)propanesulfonic acid, 3-Morpholinopropanesulfonic acid, 3-N-Morpholino propansulfonic acid, 4-Morpholinepropanesulfonic acid | |
| Identifiers | |
3D model (JSmol) | |
| ChemSpider | |
| ECHA InfoCard | 100.013.162 |
PubChem CID | |
| UNII | |
CompTox Dashboard (EPA) | |
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| Properties | |
| C7H15NO4S | |
| Molar mass | 209.26 g·mol−1 |
| Hazards | |
| GHS labelling: | |
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| Warning | |
| H315, H319, H335 | |
| P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501 | |
| Safety data sheet (SDS) | MSDS |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
Applications
MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. Usage above 20 mM in mammalian cell culture work is not recommended. MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.
See also
References
External links
- Sigma Aldrich Buffer Calculator - Useful tool to calculate weight, volume, or concentration from molecular weight.
- Recipe for MOPS buffer on OpenWetWare
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