Unique molecular identifiers (UMIs), or molecular barcodes (MBC) are short sequences or molecular tags added to DNA fragments in some next generation sequencing library preparation protocols to identify the input DNA molecule.
These tags are added before PCR amplification, and can be used to reduce errors and quantitative bias introduced by the amplification.
Applications include analysis of unique cDNAs to avoid PCR biases in iCLIP, variant calling in ctDNA, gene expression in single-cell RNA-seq (scRNA-seq) and haplotyping via linked reads[clarification needed].
This article uses material from the Wikipedia English article Unique molecular identifier, which is released under the Creative Commons Attribution-ShareAlike 3.0 license ("CC BY-SA 3.0"); additional terms may apply (view authors). Content is available under CC BY-SA 4.0 unless otherwise noted. Images, videos and audio are available under their respective licenses.
®Wikipedia is a registered trademark of the Wiki Foundation, Inc. Wiki English (DUHOCTRUNGQUOC.VN) is an independent company and has no affiliation with Wiki Foundation.